Advanced Microscopy - Equipment

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1. Nikon A1R-Si Confocal Microscope System

Nikon A1R-Si Confocal Microscope System
Taking advantages of depth discrimination or optical sectioning effects of laser scanning confocal microscopy, tomographic view of the cells or tissue can be taken like you are taking a CT image from a cell or tissue block. 3D reconstruction from the optically sliced series section can be made and viewed from different angle or animated. With multi-detectors built in the system, multi-color labeled specimens can be easily viewed or imaged in real time using the confocal microscope system.

Featured configurations:

  • Point-scanning confocal fluorescence
  • Differential Interference Contrast (DIC) imaging
  • Resonant scanning for fast imaging
  • Live imaging - short term
  • Live imaging - long term
  • Spectral imaging

Hardware components:

  • Nikon Ti-E motorized inverted microscope with Perfect Focus System
  • Nikon A1R scan head with spectral detector and resonant scanners
  • 6 laser lines:
    3 solid state lasers (405nm, 561nm and 640nm)
    Argon-krypton laser (457nm, 488nm and 514nm)
  • Tokai-Hit stage-top incubation system with 5% CO2
  • Nikon Elements version 4.30 acquisition software

Filters:

  • Confocal
Filter Set Laser Illumination Primary Dichroic Mirror Emission Filter
DAPI 404nm 405/488/561/647 450/50
FITC 488nm 405/488/561/647 525/50
TRITC 561nm 405/488/561/647 595/50
Cy5 647nm 405/488/561/647 700/75
Interference 488nm 405/488 or 405/488/561 488/10
CFP 457nm 457/514 482/35
YFP 514nm 457/514 540/30
  • Wildfield/epifluorescence
Filter Set Excitation Dichroic Emission
DAPI 395/25 400 420LP
FITC 470/40 495 525/50
TRITC 540/25 565 620/60

Instrument location:
The Nikon A1R-Si Confocal microscope is located in Room 3516, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903
 

2. Olympus Multi-field Time-Lapse Microscope System

Olympus Multi-field Time-lapse Microscope System
Live cell imaging is a field that depends largely on the development of rapid detecting device, powerful computer hardware and software, an environmental chamber which provides a suitable condition for living cells and recording images for a long time without focus shifting.

Featured configurations:

  • Phase contrast imaging
  • Motorized XY stage with multi-well plate adaptor
  • Z drive for 3D imaging
  • Multipoint and Z stacks image acquisition
  • Live imaging - short term
  • Live imaging - long term
  • Epifluorescence and dual fluorescence filter block
  • MetaMorph® NX microscopy and imaging software

Filters:

  • DsRed: Exciter = 545/30nm, Dichroic splitter = 570lp, Emission = 620/60nm
  • GFP: Exciter = 470/40nm, Dichroic splitter = 495lp, Emission = 525/50nm
  • DAPI: Exciter = 350/50nm, Dichroic splitter = 400lp, Emission = 450/50nm
  • Dual Band (2 separate exciters and emission filter) GFP/mCherry: Exciter = 470/40nm,
    Exciter = 572/35nm, Single dichroic multi-pass, Emission = 525/50nm, Emission 632/60nm

Instrument location:
The Olympus Time Lapse microscope is located in Room 2027, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903
 

3. Applied Biosciences® Arcturus XT™ Laser Capture Microdissection System

Applied Biosciences Arcturus XT Laser Capture Microdissection System
The Arcturus XT™ Laser Capture Microdissection (LCM) System is a unique microdissection instrument that combines LCM and ultraviolet (UV) laser cutting into one platform. The open, modular design enables unparalleled research flexibility and versatility. 
The solid-state IR laser delivers a gentle capture technique that preserves biomolecular integrity, and is ideal for single cells and small numbers of cells. The solid-state UV laser permits unprecedented speed and precision, and is well-suited for microdissecting dense tissue structures and for capturing large numbers of cells.

Featured configurations:

  • Life Technologies exclusive IR laser-enabled LCM and UV laser cutting for ultimate microdissection
  • Fluorescence with Nikon “noise terminator” technology
  • Phase contrast for cultured live cell isolation* (special designed dish required)
  • Use any slide preparation
  • Automated microscope controls
  • Microdissection from live images and static tiled images
  • Automatic image archiving
  • Compatible with high quality, CapSure® caps

Instrument location:
The Arcturus XT® Laser Capture Microdissecting system is located in Room 3022, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903
 

4. Zeiss Axiovert 200M Live Cell Station

Zeiss Axiovert 200M Live Cell Station
Featured configurations:

  • Living samples with temperature, CO2 environment, and multi-channel fluorescent imaging capabilities
  • Motorized XY stage with multiple-well plate adaptors
  • Z drive for 3D imaging
  • Multipoint and Z stacks image acquisition
  • Live imaging - short term
  • Live imaging - long term
  • Apotome option to remove out of focus light
  • Monochrome CCD camera 
  • AxioVision 4.8.2 imaging software

Filters:

  • Cy3, 5 Red
  • GFP
  • DAPI
  • DIC

Objectives:
5x, 10x, 20x, 40x oil immersion, 63x oil immersion

Instrument location:
The Zeiss Axiovert 200M Live Cell Station is located in Room 3516, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903
 

5. Zeiss Laser Microdissecting/UV Cutting System

Zeiss Laser Microdissecting/UV cutting System
Featured configurations:

  • Positioning Ablation Laser Micro beam (PALM) LCM system from Zeiss, which combines laser micro dissection (LM) and Laser Pressure Catapulting (LPC) is equipped with a solid state UV laser that is tightly focused and guarantee precise cutting without contacting tissue nor damaging the adjacent tissue and therefore contamination free.
  • Single cell or cluster of cells can be cut from cultured chamber slide or dish. After the desired cells/tissue has been successfully cut or damaged, the subsequent cell processing is dependent upon the molecules to be analyzed and is project specific. Downstream molecular assays include gene expression analysis, genomic profiling using microarrays, or proteomic analysis.
  • High precision motorized XY stage and monochrome CCD camera allow identification, documentation, and dissection of multiple ROI from the same tissue specimen. The microscope is also equipped with fluorescence lamp (HXP120V) and filter suitable for the identification of GFP labeled cells and fluorescent antibody labeled cell structures.

Objectives: 
5x, 10x, 20x, 40x

Instrument location:
Zeiss Laser Microdissecting/UV cutting System is located in Room 3516, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903
 

6. Essen Bioscience Continuous Live-Cell Imaging & Analysis System

Essen Bioscience Continuous Live-cell Imaging & Analysis System
Essen Bio IncuCyte Zoom is a mini scope sitting in regular cell culture incubator, which can greatly facilitate real-time assessment of cell growth and continuous long-term live cell monitoring via customized imaging protocols.

Features:

  • The ability to schedule the imaging of up to 6 plates at a time (any format size up to 384-well + large library of compatible plate types).
  • The "scan-on-demand" feature that enables additional plates to be imaged during unscheduled time.
  • Remote into the controller from your lab computer to adjust schedules, view plates, and perform analysis jobs anywhere with internet connection.
  • The high definition phase images are hard to beat and enable a number of very useful label-free analyses.

Configurations:

  • Objectives (4X, 10X, 20X),
  • High Definition Phase Contrast
  • Filter modules (Green and Red Fluorescence)
  • Software modules: IncuCyte ZOOM HD/Dual Color Filter Cube

Instrument location:
IncuCyte Zoom is located in Room 3044 Tissue Culture Room, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08903

 

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