Susan L. Cullman Laboratory for Cancer Research
164 Frelinghuysen Road
Piscataway, NJ 08854
Studying the role for the serine/threonine kinase Pak4 in cancer. Since Pak4 is upregulated in so many types of cancers, it would appear to be general mediator of oncogenic transformation, playing a vital role in many types of cancer. This makes it an attractive candidate for drug therapy for many types of cancer. In our lab we use cell lines overexpressing Pak4, as well as Pak4 knockout mice to study the role of Pak4 in oncogenic transformation in cell lines, as well as in mouse models.
Cammarano, M.S., Nekrasova, T., Noel, B., and Minden, A. (2005). PAK4 induces premature senescence via a pathway requiring p16INK4/p19ARF and MAP-Kinase signaling. Mol. Cell. Biol. 25: 9532-9542.
Berzat, A.C., Buss, J.E., Chenette, E.J., Weinbaum, C.A., Shutes, A., Der, Ch.J., Minden, A., Cox, A.D. (2005). Transforming activity of Rho family GTPase, Wrch-1, a Wnt-regulated Cdc42 omolog, is dependent on a novel carboxyl-terminal palmitoylation motif. J. Biol. Chem. 280. 3305-3365.
Li, X., and Minden, A. (2005). PAK4 functions in TNFa induced survival pathways by facilitating TRADD binding to TNFR. J. Biol. Chem. 280: 41192-41200.
Gringel, A., Walz, D., Rosenberger, G., Minden, A., Kutsche, K., Kopp, P., Linder, S. (2006). Pak4 and aPIX determine podosome size and number in macrophages through localized acting regulation. J. Cell. Physiol. 209: 568-579.
Liu, Y., Xiao, H., Tian, Y., Nekrasova, T., Hao, X., Lee, H.J., Suh, N., Yang, C.S., Minden, A. (2008). The Pak4 protein kinase plays a key role in cell survival and tumorigenesis in athymic mice. Molecular Cancer Research. 6: 1215-1224. NIHMSID: 175961
Nekrasova, T., Jobes, M.L., Tingh, J.H., Wagner, G.C., Minden, A. (2008). Targeted disruption of the Pak5 and Pak6 genes in mice leads to deficits in learning and locomotion. Developmental Biology. 322: 95-108. NIHMSID: 175947
Tian, Y., Lei, L., Cammarano, M., Nekrasova, T., Minden, A. (2009). Essential role for the Pak4 protein kinase in extraembryonic tissue development and vessel formation. Mech Dev. 126: 710-720. NIHMSID: 119335
Wong, L.E., Reynolds, A.B., Dissanayaka, N., Minden, A. (2010). p120-catenin is a binding partner and substrate for Group B Pak kinases”. J. Cell. Biochem. 110:1244-1254.
Liu, Y., Chen, N., Cui, X., Zheng, X., Deng, L., Price, S., Karantza-Wadsworth, V., and Minden, A. (2010) The protein kinase Pak4 disrupts mammary acinar architecture and promotes mammary tumorigenesis. Oncogene. 29: 5883-5894.
Tian, Y., Liang, L., Minden, A. (2011) A key role for Pak4 in proliferation and differentiation of neural progenitor cells. Dev Biol. 353: 206-216.
Nekrasova., T., and Minden, A. (2011) Pak4 is required for regulation of cell cycle regulatory protein p21, and for control of cell cycle progression. J Cell. Biochem. 112:1795-1806.
Nekrasova, T. and Minden, A. (2012). Role for p21-activated kinase Pak4 in development of the mammalian heart. Transgenic Research. 21:797-811.
So, J.Y., Lee, H.J., Kramata, P., Minden, A., Suh, N. (2012) Differential expression of key signaling proteins in MCF10 cell lines, a human breast cancer progression model. Mol. Cell. Pharmacol. 4:31-40.
Strochlic, T.I, Concilio, S., Viaud, J., Eberwine, R., Wong, L.E., Minden, A., Turk, B., Plomann, M., Peterson, J. (2012) Identification of neuronal substrates implicates Pak5 in synaptic vesicle trafficking. Proc. Natl. Acad Sc. 109: 4116-4121.
Coste, O., Möser, C.V., Sisignano, M., Kynast, K.L.; Minden, A. Geisslinger, G., Niederberger, E. (2012) The p21-activated kinase PAK 5 is involved in formalin-induced nociception through regulation of MAP-Kinase signaling and formalin-specific receptors. Behavioral Brain Research. 234:121-128.