IL-10 and IL-10 receptors
• Chronic Lymphocyte Leukemia (CLL)
The majority of my research effort has been in the identification of clonal B-1 cells in both humans with CLL and in the NZB mouse model of CLL. The predominant detection method is flow cytometry analysis of unique surface markers which my lab has been employing and first reported the mouse counterpart to B-1 clones in 1981. With collaborators, we found that the development of CLL in NZB mice was liked to a mutation in the micorRNA loci mir-15a/16. My expertise in microRNA analysis and flow cytometry is the basis for a recent NIH R01 award. This work involves sorting cells which have been transduced with vectors expressing both fluorescent proteins and delivered genes (such as the microRNAs). The laboratory has established NZB derived ES cells and iPS cells. Constructs have been made to correct the NZB mutation or introduce the NZB mutation into normal ES cells and transgenic mice are being developed.