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Flow Cytometry/Cell Sorting - Equipment

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New Brunswick Site

Flow Cytometry / Cell Sorting New BrunswickBD Biosciences Influx High Speed Cell Sorter
This is an open-architecture, high-speed cell sorter for rapid separation of large numbers of specific cell populations with high purity, recovery, and yield. Using up to four different lasers, cell sorting is based on acquisition of up to 14 parameters, including twelve fluorescence parameters. The 488, 561, 640 nm and UV solid-state lasers provide the ability to detect a large array of fluorophores. The Influx can sort cells aseptically at rates up to 20,000 cells per second, or higher (upper limit of 100,000 events/sec), depending on the cell size and type. In addition, four different subpopulations can be collected simultaneously. The optical detection system has interchangeable filters for custom applications, allowing optimization to the needs of researchers. Cells can be sorted into various configurations of collection vessels, including automated single-cell sorting into almost any type of plate configuration, including custom layouts. Index sorting is available to track the identity of individually-sorted cells. The Influx is enclosed in a class II-certified biohazard containment cabinet with an aerosol management system allowing for safe sorting of human and primate cells. The system can be easily sterilized for aseptic sorting. Additionally, for specialty applications, the entire sample handling system can be removed and autoclaved or exchanged for a new gamma-irradiated unit. It is equipped with five nozzle sizes (70, 86, 100, 140, and 200 microns) and temperature control capability. Available by appointment with Flow staff.

Flow Cytometry / Cell Sorting New Brunswick SiteBD Biosciences LSRII Flow Cytometer
This is an advanced bench top instrument with three fixed alignment lasers, including 405, 488, and 640 nm laser excitation sources. It is capable of up to 10-color detection of a wide variety of fluorophores and is equipped to run individual tubes using FACSDiVa software. Available 24/7 to trained users.

BD FACS Canto Flow Cytometer
This is a user-friendly flow cytometer that provides reliable performance, accuracy, and ease-of-use. Equipped with two spatially separated lasers (488 and 640 nm) and six fluorescence detection channels, in addition to FSC and SSC, this instrument is capable of handling the majority of multi-color assays. Available 24/7 to trained users.

Beckman-Coulter Cytomics FC500 Flow Cytometer
This user-friendly analytical instrument is equipped with two lasers (488 and 640 nm) and five fluorescence detection channels, in addition to FSC and SSC, for simultaneous measurement of five different fluorescent probes. This instrument is capable of handling many of the simpler assays. Available 24/7 to trained users.

 

Princeton Site

Flow Cytometry / Cell Sorting PrincetonBD Biosciences LSRII w/ HTS Flow Cytometer
This is a user-friendly five-laser fixed alignment bench top instrument with 355, 405, 488, 561 and 640 nm laser excitation sources.  It is capable of up to 14-color detection of a wide variety of fluorochromes and is equipped to run individual tubes or 96- and 384-well plates with the high-throughput (HTS) automated microsampler.  Available by appointment with Flow staff and to trained users 24/7.

Union Biometrica COPAS BioSort
This large particle cytometer is capable of analyzing, sorting and dispensing cells and organisms of sizes 40-175 microns. It is optimal for use with all life-cycle stages of C. elegans, and with small embryoid bodies and cell clusters. It features a 488 nm laser excitation source-with five parameter (three-color) acquisition; a ReFlx system for sampling directly from 96-well plates; and the Profiler II option that allows for the spatial mapping of fluorescence signals along the length of the organism or cell clusters.  Available by appointment with Flow staff and to trained users 24/7.

Flow Cytometry / Cell Sorting PrincetonBio-Rad S3e Cell Sorter
This two-laser (488 & 561nm), four-color, user-friendly benchtop cell sorting system is capable of 6-parameter simultaneous acquisition.  It is very useful for the isolation of cells expressing fluorescent protein reporters such as GFP, YFP, mRFP, mCherry, etc or cells labeled with markers such as antibodies labeled with FITC, PE and PE-tandems.  It can sort into two tubes or 8-well strip plates and has temperature control options.  This system has automated alignment, sort setup, and fluidics level monitoring features that allow for independent use by trained students.  Available by appointment with Flow staff and to trained users 24/7.

BD Biosciences FACSAria Fusion Cell Sorter
This custom-built five laser (355, 405, 488, 561, and 640nm), fixed alignment 20-parameter (17-color) fluorescence activated cell sorter was acquired in 2017.  The system is housed in a Baker Class II biosafety cabinet for safer, sterile sorting of BSL1 and BSL2 level materials.  It is equipped with four nozzle sizes (70, 85, 100, and 130 microns), tube and plate sorting capabilities, including index sorting to track phenotypes of single-sorted cells, and temperature control options. This cell sorter is available by appointment with Flow staff.

 

 

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