Helmut Zarbl, PhD
Professor of Environmental and Occupational Medicine
Phone: (848) 445-2354
Assistant: Kimi Nakata
Research Program Alignment
Membership Type: Full
- Toxicogenomics and functional genomics, genetics, molecular carcinogenesis, molecular and cellular biology, and toxicology. Specifically: molecular mechanisms of chemical carcinogenesis and the genetic basis for differential susceptibility to mammary carcinogenesis using both animal and in vitro model systems. Studies in the rat model have included analysis of oncogene activation, mechanisms of signal transduction, and genetic linkage analysis to identify mammary tumor susceptibility genes. Also have used toxicogenomics to dissect mechanisms of mechanism carcinogenesis, tumor progression and chemoprevention. Studies in the area of toxicogenomics include the development and application of standards for DNA microarray experiments, and phenotypic anchoring of response of human cells, model organisms (yeast) and target organs (rodents) to toxicants, providing insights into dose and temporal responses, as well as mechanisms of action. Also actively involved in technology development, including my patented work on RNAi and its application to the development of novel platforms (with Engineering Arts, Inc.) for functional genomics and biomarker screening.
Ricicki, E. M., Luo, W., Fan, W., Zhao, L.P., Zarbl, H. and Vouros. P. (2006) Quantification of N-(deoxyguanosin-8-yl)-4-aminobiphenyl adducts in human lymphoblastoid TK6 cells dosed with N-hydroxy-4-acetylaminobiphenyl and their relationship to mutation, toxicity, and gene expression profiling. Analytical Chemistry 78(18):6422-32.
Mikheev, A. M., Mikheeva, S. A., Rostomily, R., and Zarbl, H. (2007). Dickkopf-1 activates cell death in MDA-MB435 melanoma cells. Biochemical and Biophysical Research Communications 352, 675-680.
Olsavsky, K. M., Page, J. L., Johnson, M. C., Zarbl, H., Strom, S. C., and Omiecinski, C. J. (2007). Gene expression profiling and differentiation assessment in primary human hepatocyte cultures, established hepatoma cell lines, and human liver tissues. Toxicology and Applied Pharmacology 222, 42-56.
Page, J. L., Johnson, M. C., Olsavsky, K. M., Strom, S. C., Zarbl, H., and Omiecinski, C. J. (2007). Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci 97, 384-397.
Beyer, R.P., Fry, R.C., Lasarev, M.R., McConnachie, L.A., Meira, L.B., Palmer, V., PowellC.L., Ross, P.K., Bammler, T.K., Bradford, B.U., Cranson, A.B., Cunningham, M.L., Fannin, R.D., Higgins, G.M., Hurban, P., Kayton, R.J., Kerr, K.F., Kosyk, O., Lobenhofer, E.K., Sieber, S.O., Vliet, P.A., Weis, B.K., Wolfinger, R., Woods, C.G., Freedman, J.H., Linney, E., Kaufmann, W.K., Kavanagh, T.J., Paules, R.S., Rusyn, I., Samson, L.D., Spencer, P., Suk, W., Tennant, R.J., Zarbl, H. (and other Members of the Toxicogenomics Research Consortium). (2007). Multi-center study of acetaminophen hepatotoxicity reveals the importance of biological endpoints in genomic analyses. Toxicological Sciences 99(1):326-337.
Zarbl, H. Toxicogenomic Analyses of Genetic Susceptibility to Mammary Gland Carcinogenesis in Rodents: Implications for Human Breast Cancer. Breast Disease 28: 87-105, 2007.
Ren, X., Zhang, X., Kim, A. S., Mikheev, A. M., Fang, M., Sullivan, R.Bumgarner, R., Zarbl, H. Comparative Genomics of Susceptibility to Mammary Carcinogenesis among Inbred Rat Strains: Role of Reduced Prolactin Signaling in Resistance of the Copenhagen Strain. Carcinogenesis 29(1): 177-185 2007. ePub 2007/10/06
Mikheev, A.M., Mikheeva, S.A., Rostomily, R., Swisshelm, K., and Zarbl, H. (2008). Dickkopf-1 mediated tumor suppression in human breast carcinoma cell lines. Breast Cancer Research and Treatment. 112(2):263-273. ePub 2007/12/25
Zhang, X and Zarbl, H. Chemopreventive Doses of Methylselenocysteine Alter Circadian Rhythm in Rat Mammary Tissue but Not Liver. (2008). Cancer Prevention Research 2(1):119-127.
Sudo, H., Li-Sucholeiki, X.C., Marcelino, L.A., Gruhl, A.N., Herrero-Jimenez, P., Zarbl, H., Willey, J.C., Furth, E.E., Coller, H., Kurzweil, R., Ekstrom, P.O., Morgenthaler, S., Gostjeva, E.V., and Thilly, W.G. (2008) Fetal-juvenile origins of point mutations in the adult human tracheal-bronchial epithelium: absence of detectable effects of age, gender or smoking status. Mutation Research 646:15-40, 2008.
Zarbl. H., Gallo,M.A., Glick, J., Yeung, K.Y. and Vouros, P. (2010) The Vanishing Zero Revisited: Thresholds in the Age of Genomics. Chemico-Biological Interactions 184:273-278, 2010.
Ming Zhu Fang, Xun Zhang and Helmut Zarbl. (2010). Methylselenocysteine Inhibits Carcinogenesis by Enhancing Circadian Expression of Melatonin Receptor 1α and Estrogen Receptor β in Rat Mammary Epithelial Cells.
Cancer Prevention Research 3(5): 640-652, 2010. PubMed # 20424134 NIHMSID # 181679, Publ.ID: CAPR-09-0170.
Glen E Kisby, Rebecca C Fry, Michael Lasarev, Theodor K Bammler, Richard P Beyer, Monica Cherchwell, Daniel Doerge, Lisiane B Meira, Valerie S Palmer, Ana Luiza Ramos-Crawford, XueFeng Ren, Robert C Sullivan, Terrance Kavanagh, Leona D Samson, Helmut Zarbl, Peter Spencer. (2010) The Cycad Genotoxin MAM Modulates Cellular Pathways Involved in Cancer and Neurodegenerative Disease in a DNA Damage-Linked Manner. PLoS ONE 6(6): e20911. doi:10.1371/journal.pone.0020911
Elisa V. Bandera, Urmila Chandran, Brian Buckley, Bozena Winnik, Yong Lin, Sastry Isukapalli, Ian Marshall, Melony King, and Helmut Zarbl. (2011). Urinary mycoestrogens, body size and breast development in New Jersey girls. Science of the Total Environment 409:5221-5227. PMID 21975003
Jessica C. Graham and Helmut Zarbl. (2012). Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-associated Cervical Cancer Cells. PLoS ONE 7(4):1-9. e36103