Instrumentation New Brunswick:
Cytek Aurora: Cytek Aurora is a full spectrum flow cytometer with 4 lasers, 54 detectors, vacuum fluidics, small particle detection using Violet laser SSC, and capable of 40+ color detection. The Aurora system’s implementation of full-spectrum cytometry enables autofluorescence extraction to improve data clarity, which is critical for immune cells isolated from tumor tissues.
Cytek Aurora High-Speed Cell Sorter: provides benefits of full-spectrum profiling along with high speed up to 6-way sorting, customizable nozzle settings and sort modes, automated drop delay, and sort stream monitoring.
Invitrogen Attune NxT Flow Cytometers: The Attune systems are advanced, high throughput, acoustic focusing, benchtop flow cytometers equipped with 4 laser/14 colors and 3 laser/11 colors configuration operating on Attune™ NxT Software for data acquisition and analysis.
Luminex 200: performs multiplex analysis of up to 100 analytes in as little as 50 microliters of serum/plasma/cell culture.
xCELLigence Real-Time Cell Assay: The Agilent xCELLigence Real-Time Cell Analysis (RTCA) SP instrument uses label-free cellular impedance to evaluate the interaction of CAR-T cells and TILs with tumor cells, providing additional information about tumor escape and immune cell exhaustion.
NanoString nCounter Max: performs multiplex gene expression analysis in humans or mice with 770 genes from different immune cell types, common checkpoint inhibitors, CT antigens, and genes covering both the adaptive and innate immune response.
Instrumentation Princeton:
FACSymphony A3 flow cytometer: is a state-of-the-art flow cytometer equipped with 5 laser lines and capable of 23-color detection utilizing the FACSDiva software. It has low-noise VPX electronics for improved fluorescence detection sensitivity and is equipped to run individual tubes or in HTS mode for automated sampling from 96- and 384-well plates. It is available to trained users 24/7 for a wide variety of assays.
S3e cell sorter: The S3e Cell Sorter is a user-friendly automated cell sorter with real-time monitoring and smart features.
FACSAria Fusion cell sorter: This five laser (355, 405, 488, 561, and 640nm), 17-color fluorescence-activated cell sorter is housed in a Baker Class II biosafety cabinet for safer, sterile sorting of BSL1 and BSL2 level materials. This cell sorter is available by appointment with core facility staff and is critical to several labs, including the Kang (CMI) lab studies of mammary gland stem cells and metastasis.
Last updated 07/01/2022