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Genome Editing Services

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  • CRISPR-Cas9-mediated genome editing in mice (C57BL/6J, NSG, other backgrounds): 
    • Gene knockout/knockin
    • Epitope tagging
    • Fluorescent protein tagging
    • Generation of conditional knockout models 
    • Knock-In to safe harbor Loci (Rosa26, H11)
  • CRISPR-Cas9-mediated genome editing in cell lines (HEK293, HeLa, NIH3T3)
  • Transgenic mouse generation
  • Blastocyst microinjection of gene targeted ES cells
  • Mouse line cryopreservation
  • Mouse line recovery/rederivation (live/cryopreserved embryos and IVF)
  • Molecular biology services
    • Plasmid DNA and BAC DNA purification
    • Genotyping assay design
    • Genotype analysis of founder mice